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Institut de minéralogie, de physique des matériaux et de cosmochimie
UMR 7590 - UPMC/CNRS/IRD/MNHN

Structure and dynamics of signalling proteins Group leader C. Vénien-Bryan

Many signals in the cell are conveyed by interacting protein molecules. How do protein-protein interactions lead to a response? The most likely explanation is through changes in structure. We study protein-protein interactions in the control of signalling processes using cryo-electron microscopy and combining the results with information from X-ray diffraction and biophysical studies.

 

Ion Channels

The regulation of the flow of potassium ions into cells is important in the propagation of the neuronal action potential, cell volume regulation and muscle contraction. Potassium channels are controlled by pore gating: the channel can exist in either an open or a closed state. Our research is focussed on understanding the intimate relationship between ion channel structure and function. The objective is to understand the molecular mechanism at an atomic level.

We are focusing on the inwardly-rectifying family of potassium channels or ‘Kir’ channels, more precisely Kirbac3.1 channel but also the eucaryotic Kir2.1

channel.

 

 

Genome integrity:

Genome integrity is maintained with various DNA repair pathways. At the center of the DNA damage Fanconi Anemia repair pathway is the FANCD2/FANCI complex. 

We are using cryo electron microscopy and image analysis for studying this complex machinary.  Using a combination of molecular biology, cell biology, biochemistry and structural biology, we have identified the fork-like tower domain in the C-terminus of FANCD2 which is required for its complete functions in the DNA interstrand cross-link repair. Interestingly, several disease-causing mutations lie within this region, underscoring the importance of this domain. (collaboration Dr M Cohn, University of Oxford)

 

 

The CRACAM robot:  Two-dimensional crystallization of membrane protein.

Membrane proteins are key cellular components that perform essential functions. They are major therapeutic targets.  Electron crystallography can provide structural experimental information at atomic scale for membrane proteins forming two-dimensional crystals.  We have created a fully automated robot called CRACAM which is able to produce 2D crystals of membrane proteins either directly in the bulk of the solution or under a lipid monolayer at the air:water interface. This robot is versatile and can be used also for immobilising various macromolecules at the air-water interface.

 

 Publications

Charline Fagnen (charline.fagnen @ impmc.upmc.fr) - 28/08/17

Dans cette rubrique

Traductions :

    Egalement dans la rubrique

      Mesurer l’importance des effets quantiques avec des ondes sonores : l’élasticité de l’hydrogène solide à haute pression

      Le principe d’Heisenberg de la mécanique quantique interdit la détermination simultanée de la position et de la vitesse. Cela se traduit par des vibrations résiduelles des atomes même à température nulle. Aujourd’hui encore, l’importance de la contribution des effets quantiques avec l’augmentation de...

      » Lire la suite

      Contact

      Guillaume Fiquet (Guillaume.Fiquet @ impmc.upmc.fr)

      Directeur de l'institut

      33 +1 44 27 52 17

       

      Nalini Loret (Nalini.Loret @ impmc.upmc.fr)

      Attachée de direction

      33 +1 44 27 52 17

       

      Dany Thomas-Emery (danielle.thomas @ impmc.upmc.fr)

      Gestion du personnel

      33 +1 44 27 74 99

       

      Danielle Raddas (cecile.duflot @ impmc.upmc.fr)

      Gestion financière

      33 +1 44 27 56 82

       

      Cécile Duflot (cecile.duflot @ impmc.upmc.fr)

      Chargée de communication

      33 +1 44 27 46 86

       

      Expertise de météorites

       

      Expertise de matériaux et minéraux

       

      Adresse postale

      Institut de minéralogie, de physique des matériaux et de cosmochimie - UMR 7590

      Université Pierre et Marie Curie - 4, place Jussieu - BC 115 - 75252 Paris Cedex 5

       

      Adresse physique

      Institut de minéralogie, de physique des matériaux et de cosmochimie - UMR 7590

      Université Pierre et Marie Curie - 4, place Jussieu - Tour 23 - Barre 22-23, 4e étage - 75252 Paris Cedex 5

       

      Adresse de livraison

      Accès : 7 quai Saint Bernard - 75005 Paris, Tour 22.

      Contact : Antonella Intili : Barre 22-23, 4e étage, pièce 420, 33 +1 44 27 25 61

       

       

      Fax : 33 +1 44 27 51 52

      L'IMPMC en chiffres

      L'IMPMC compte environ 195 personnes dont :

       

      • 40 chercheurs CNRS
      • 46 enseignants-chercheurs
      • 19 ITA CNRS
      • 15 ITA non CNRS
      • 50 doctorants
      • 13 post-doctorants
      • 12 bénévoles

       

       Chiffres : janvier 2016